MGM101/YJR144W Summary

MGM101/YJR144W Summary

Standard Name	MGM101 1
Systematic Name	YJR144W
Alias	MGM9
Feature Type	ORF, Verified
Description	Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage (1, 2, 3, 4, 5)
Name Description	Mitochondrial Genome Maintenance 1

Chromosomal Location	ChrX:700882 to 701691 \| ORF Map \| GBrowse

Gene Ontology Annotations All MGM101 GO evidence and references

	View Computational GO annotations for MGM101
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	DNA repair (IMP) DNA strand renaturation (IDA) interstrand cross-link repair (IGI) recombinational repair (IMP)
Cellular Component
Manually curated	mitochondrial nucleoid (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All MGM101 Phenotype evidence and references

Classical genetics
conditional	UV resistance: decreased gamma ray resistance: decreased mitochondrial genome maintenance: absent mitochondrial genome maintenance: abnormal oxidative stress resistance: decreased respiratory growth: absent
null	mitochondrial genome maintenance: absent respiratory growth: absent
Large-scale survey
null	cell size: decreased glycogen accumulation: decreased glutathione excretion: increased competitive fitness: decreased dessication resistance: decreased fermentative growth: decreased rate filamentous growth: decreased haploinsufficient heat sensitivity: increased invasive growth: absent metal resistance: increased mitochondrial morphology: abnormal resistance to formaldehyde: decreased resistance to elesclomol-Cu: decreased resistance to trichothecene: increased resistance to citric acid: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased respiratory growth: decreased rate respiratory growth: absent utilization of carbon source: decreased utilization of carbon source: absent vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	filamentous growth: increased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MGM101 Interaction evidence and references

	214 total interaction(s) for 178 unique genes/features.
Physical Interactions	Affinity Capture-MS: 33 Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Reconstituted Complex: 1
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 101 Phenotypic Enhancement: 1 Positive Genetic: 56 Synthetic Growth Defect: 15
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MGM101 Protein evidence and references

Localization	Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:700882 to 701691 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..810	700882..701691	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003905

References cited on this page View Complete Literature Guide for MGM101

1)	Chen XJ, et al. (1993) MGM101, a nuclear gene involved in maintenance of the mitochondrial genome in Saccharomyces cerevisiae. Nucleic Acids Res 21(15):3473-7
2)	Meeusen S, et al. (1999) Mgm101p is a novel component of the mitochondrial nucleoid that binds DNA and is required for the repair of oxidatively damaged mitochondrial DNA. J Cell Biol 145(2):291-304
3)	Zuo X, et al. (2007) A functional core of the mitochondrial genome maintenance protein Mgm101p in Saccharomyces cerevisiae determined with a temperature-conditional allele. FEMS Yeast Res 7(1):131-40
4)	Mbantenkhu M, et al. (2011) Mgm101 is a Rad52-related protein required for mitochondrial DNA recombination. J Biol Chem 286(49):42360-70
5)	Ward TA, et al. (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884