MGA2/YIR033W Summary Help

Standard Name MGA2 1
Systematic Name YIR033W
Feature Type ORF, Verified
Description ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication (2, 3, 4)
Name Description Multicopy suppressor of GAm1 (snf2) 1
Chromosomal Location
ChrIX:416124 to 419465 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MGA2 GO evidence and references
  View Computational GO annotations for MGA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
390 total interaction(s) for 227 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Negative Genetic: 139
  • Phenotypic Enhancement: 28
  • Phenotypic Suppression: 99
  • Positive Genetic: 48
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 10
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,113
Molecular Weight (Da) 127,053
Isoelectric Point (pI) 4.89
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:416124 to 419465 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3342 416124..419465 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001472
References cited on this page View Complete Literature Guide for MGA2
1) Zhang S, et al.  (1997) Genetic redundancy between SPT23 and MGA2: regulators of Ty-induced mutations and Ty1 transcription in Saccharomyces cerevisiae. Mol Cell Biol 17(8):4718-29
2) Chellappa R, et al.  (2001) The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem 276(47):43548-56
3) Shcherbik N, et al.  (2003) Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90. Curr Biol 13(14):1227-33
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61