MET4/YNL103W Summary

MET4 BASIC INFORMATION

Standard Name	MET4 1
Systematic Name	YNL103W
Feature Type	ORF, Verified
Description	Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p (2)
Name Description	METhionine requiring 1

GO Annotations	All MET4 GO evidence and references
	View Computational GO annotations for MET4
Molecular Function
Manually curated	transcription coactivator activity (IDA)
Biological Process
Manually curated	positive regulation of transcription from RNA polymerase II promoter (IDA) response to arsenic (IDA) response to cadmium ion (IDA) sulfur amino acid metabolic process (IMP)
Cellular Component
Manually curated	nucleus (IPI)

Regulatory Role
Binding motifs	MET4 Transcription Factor Binding Sites and References
	RMMAWSTGKSGYGSC

Mutant Phenotype	All MET4 Phenotype details and references
Classical genetics
null	auxotrophy resistance to acetaldehyde: decreased
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cell size: increased cellular morphology: abnormal
Large-scale survey
null	inviable vegetative growth: decreased
overexpression	vegetative growth: decreased

Interactions	MET4 All interactions details and references
	45 total interaction(s) for 23 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 9 Reconstituted Complex: 6 Two-hybrid: 13
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 1 Synthetic Rescue: 2

Sequence Information

ChrXIV:427737 to 429755 | |

Genetic position: -76 cM

Last Update

Coordinates: 2005-11-07 | Sequence: 2006-11-09

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2019	427737..429755	2005-11-07	2006-11-09

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005047

MET4 RESOURCES

Click on map for expanded view

SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for MET4

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for MET4]

1)	Masselot M and De Robichon-Szulmajster H (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2)	Blaiseau PL and Thomas D (1998) Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. EMBO J 17(21):6327-36

SGD^tm pages Database Copyright © 1997-2010 The Board of Trustees of Leland Stanford Junior University. Permission to use the information contained in this database was given by the researchers/institutes who contributed or published the information. Users of the database are solely responsible for compliance with any copyright restrictions, including those applying to the author abstracts. Documents from this server are provided "AS-IS" without any warranty, expressed or implied. The SGD project at Stanford University is supported by a Genome Research Resource Grant from the US National Human Genome Research Institute, part of the US National Institutes of Health.