MET32/YDR253C Summary Help

Standard Name MET32 1
Systematic Name YDR253C
Feature Type ORF, Verified
Description Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description METhionine requiring 1
Chromosomal Location
ChrIV:964565 to 963990 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MET32 GO evidence and references
  View Computational GO annotations for MET32
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Targets 409 genes
Regulators 5 genes
Classical genetics
Large-scale survey
58 total interaction(s) for 40 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Negative Genetic: 28
  • Phenotypic Enhancement: 2
  • Positive Genetic: 5
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 191
Molecular Weight (Da) 21,518
Isoelectric Point (pI) 10.67
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:964565 to 963990 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..576 964565..963990 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002661

MET gene expression is driven by large multisubunit complexes which assemble on the 5' upstream regions of the MET genes. These complexes contain Met4p, the single activator required for the transcriptional regulation of the sulfur amino acid pathway, and, depending on the gene, different combinations of the auxiliary factors Met28p, Cbf1p, Met31p, or Met32p (4, 5, 6, 7). Met31p and Met32p are homologous (46% identical) zinc finger-containing proteins, each having an amino-terminal zinc finger of the CC/HH type, and a carboxy-terminal zinc finger of the CC/HC type (1, 8). Met32p, itself devoid of any intrinsic transcription activation function, binds the upstream element 5'-AAACTGTGG-3', which is found in the promoters of some of the methionine biosynthetic genes, and acts in recruiting Met4p to the DNA (1, 8, 9, 6). The function of Met32p during the transcriptional regulation of the sulfur network varies, however, from one gene to another, as it seems to act as a negative trans-regulatory factor at the MET17 promoter region, but as an essential positive effector at the MET3, MET14, and MET30 promoter regions (1, 8, 7). In addition, Met32p is also responsible for regulating GSH1 expression in response to cadmium (9), and may play a role in coregulating genes involved in copper and iron metabolism (10).

Last updated: 2003-05-15 Contact SGD

References cited on this page View Complete Literature Guide for MET32
1) Blaiseau PL, et al.  (1997) Met31p and Met32p, two related zinc finger proteins, are involved in transcriptional regulation of yeast sulfur amino acid metabolism. Mol Cell Biol 17(7):3640-8
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) McIsaac RS, et al.  (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007
4) Blaiseau PL and Thomas D  (1998) Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. EMBO J 17(21):6327-36
5) Craig KL and Tyers M  (1999) The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol 72(3):299-328
6) Patton EE, et al.  (2000) SCF(Met30)-mediated control of the transcriptional activator Met4 is required for the G(1)-S transition. EMBO J 19(7):1613-24
7) Rouillon A, et al.  (2000) Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex. EMBO J 19(2):282-94
8) Thomas D and Surdin-Kerjan Y  (1997) Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61(4):503-32
9) Dormer UH, et al.  (2000) Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. J Biol Chem 275(42):32611-6
10) Moler EJ, et al.  (2000) Integrating naive Bayes models and external knowledge to examine copper and iron homeostasis in S. cerevisiae. Physiol Genomics 4(2):127-135
11) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
12) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87