MET31/YPL038W Summary Help

Standard Name MET31 1
Systematic Name YPL038W
Feature Type ORF, Verified
Description Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description METhionine requiring 1
Chromosomal Location
ChrXVI:480535 to 481068 | ORF Map | GBrowse
Gene Ontology Annotations All MET31 GO evidence and references
  View Computational GO annotations for MET31
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Targets 24 genes
Regulators 3 genes
Large-scale survey
51 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 16

Genetic Interactions
  • Negative Genetic: 23
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 177
Molecular Weight (Da) 19,557
Isoelectric Point (pI) 9.98
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:480535 to 481068 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..534 480535..481068 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005959

MET31 encodes a DNA-binding protein involved in the transcriptional control of genes involved in sulfur metabolism (1, 4, 5). Transcription of these genes is driven by large multisubunit complexes that assemble on their 5' upstream regions. Depending on the target, different combinations of the Cbf1p, Met28p, Met31p, and Met32p factors are assembled and tether the transcription factor Met4p to the DNA (6, 4, 5). Met31p, itself devoid of any intrinsic transcription activation function, appears not to act during the transcriptional regulation of all of the MET genes, but recruits Met4p to the promoters of some of the methionine biosynthetic genes, and is required for their expression (1, 7, 4). Met31p and Met32p are highly-related (46% identical) zinc finger-containing proteins, each containing an amino-terminal zinc finger of the CC/HH type, and a carboxy-terminal zinc finger of the CC/HC type (1, 8). Both proteins recognize and bind the upstream element 5'-AAACTGTGG-3', and the binding of Cbf1p in the vicinity of this motif enhances this affinity (9). The function of Met31p during the transcriptional regulation of the sulfate assimilation pathway varies from one gene to another, as Met31p seems to function as a negative regulatory factor at the MET25 promoter region, but as an essential positive effector at the MET3 and MET14 promoter regions (1, 8). The function of Met31p is not restricted to these pathways, however, as it is also involved in regulating GSH1 expression in response to cadmium (7), and may play a role in coregulating genes involved in copper and iron metabolism (10).

Last updated: 2003-05-27 Contact SGD

References cited on this page View Complete Literature Guide for MET31
1) Blaiseau PL, et al.  (1997) Met31p and Met32p, two related zinc finger proteins, are involved in transcriptional regulation of yeast sulfur amino acid metabolism. Mol Cell Biol 17(7):3640-8
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) McIsaac RS, et al.  (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007
4) Patton EE, et al.  (2000) SCF(Met30)-mediated control of the transcriptional activator Met4 is required for the G(1)-S transition. EMBO J 19(7):1613-24
5) Rouillon A, et al.  (2000) Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex. EMBO J 19(2):282-94
6) Craig KL and Tyers M  (1999) The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol 72(3):299-328
7) Dormer UH, et al.  (2000) Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. J Biol Chem 275(42):32611-6
8) Thomas D and Surdin-Kerjan Y  (1997) Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61(4):503-32
9) Blaiseau PL and Thomas D  (1998) Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. EMBO J 17(21):6327-36
10) Moler EJ, et al.  (2000) Integrating naive Bayes models and external knowledge to examine copper and iron homeostasis in S. cerevisiae. Physiol Genomics 4(2):127-135
11) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87