MEK1/YOR351C Summary Help

Standard Name MEK1 1
Systematic Name YOR351C
Alias MRE4 2
Feature Type ORF, Verified
Description Meiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description MEiotic Kinase 1
Chromosomal Location
ChrXV:996511 to 995018 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 175 cM
Gene Ontology Annotations All MEK1 GO evidence and references
  View Computational GO annotations for MEK1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 101 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
227 total interaction(s) for 144 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 35
  • Reconstituted Complex: 28
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 104
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 7
  • Positive Genetic: 14
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 497
Molecular Weight (Da) 56,849
Isoelectric Point (pI) 7.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:996511 to 995018 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 175 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1494 996511..995018 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005878
SUMMARY PARAGRAPH for MEK1

Red1p, Hop1p, and Mek1p are components of the axial element protein cores in synaptonemal complexes (7). Synaptonemal complexes are found at synapses between homologous chromosomes during meiosis, and form when sister chromatids condense upon axial elements (7). Maintenance of appropriate stoichiometry between Red1p, Hop1p, and Mek1p is important for effective chromosome segregation and for the meiotic recombination checkpoint (8). Red1p, Hop1p and Mek1p are also necessary for normal levels of double-strand break (DSB) formation (9), and are required to ensure that crossovers occur between homologous chromosomes and not between sister chromatids (8). Red1p is a multifunctional protein required for maximum activity of Mek1p, interaction between Mek1p and Hop1p, sister chromatid cohesion, establishment of interhomolog bias at double Holliday junctions, and for proper timing of the first meiotic division (8, 10, 11, 12).

Last updated: 2005-10-14 Contact SGD

References cited on this page View Complete Literature Guide for MEK1
1) Rockmill B and Roeder GS  (1991) A meiosis-specific protein kinase homolog required for chromosome synapsis and recombination. Genes Dev 5(12B):2392-404
2) Leem SH and Ogawa H  (1992) The MRE4 gene encodes a novel protein kinase homologue required for meiotic recombination in Saccharomyces cerevisiae. Nucleic Acids Res 20(3):449-57
3) Bailis JM, et al.  (2000) Bypass of a meiotic checkpoint by overproduction of meiotic chromosomal proteins. Mol Cell Biol 20(13):4838-48
4) Bailis JM and Roeder GS  (2000) Pachytene exit controlled by reversal of Mek1-dependent phosphorylation. Cell 101(2):211-21
5) Niu H, et al.  (2007) Mek1 kinase is regulated to suppress double-strand break repair between sister chromatids during budding yeast meiosis. Mol Cell Biol 27(15):5456-67
6) Chuang CN, et al.  (2012) Mek1 stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during yeast meiosis. Nucleic Acids Res 40(22):11416-27
7) de Los Santos T, et al.  (2001) A role for MMS4 in the processing of recombination intermediates during meiosis in Saccharomyces cerevisiae. Genetics 159(4):1511-25
8) Wan L, et al.  (2004) Mek1 kinase activity functions downstream of RED1 in the regulation of meiotic double strand break repair in budding yeast. Mol Biol Cell 15(1):11-23
9) Prieler S, et al.  (2005) The control of Spo11's interaction with meiotic recombination hotspots. Genes Dev 19(2):255-69
10) Malone RE, et al.  (2004) The signal from the initiation of meiotic recombination to the first division of meiosis. Eukaryot Cell 3(3):598-609
11) Woltering D, et al.  (2000) Meiotic segregation, synapsis, and recombination checkpoint functions require physical interaction between the chromosomal proteins Red1p and Hop1p. Mol Cell Biol 20(18):6646-58
12) Zierhut C, et al.  (2004) Mnd1 is required for meiotic interhomolog repair. Curr Biol 14(9):752-62