Standard Name	MED8 1
Systematic Name	YBR193C
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description	MEDiator complex 1

Chromosomal Location	ChrII:609753 to 609082 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for MED8
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA) RNA polymerase II transcription corepressor activity (IPI) structural molecule activity (IMP) TBP-class protein binding (IDA)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IDA) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA)

Classical genetics
null	inviable
reduction of function	heat sensitivity: increased vegetative growth: decreased
Large-scale survey
null	haploinsufficient inviable resistance to ethanol: decreased
overexpression	silencing: abnormal
reduction of function	competitive fitness: decreased
repressible	chromosome/plasmid maintenance: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	306 total interaction(s) for 173 unique genes/features.
Physical Interactions	Affinity Capture-MS: 64 Affinity Capture-RNA: 1 Affinity Capture-Western: 37 Co-crystal Structure: 2 Co-purification: 22 PCA: 1 Reconstituted Complex: 7 Two-hybrid: 10
Genetic Interactions	Negative Genetic: 106 Positive Genetic: 9 Synthetic Growth Defect: 45 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:609753 to 609082 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..672 609753..609082 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000397