MED8/YBR193C Summary Help

Standard Name MED8 1
Systematic Name YBR193C
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description MEDiator complex 1
Chromosomal Location
ChrII:609753 to 609082 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MED8 GO evidence and references
  View Computational GO annotations for MED8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
conditional
null
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
309 total interaction(s) for 173 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 64
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 37
  • Co-crystal Structure: 2
  • Co-purification: 22
  • PCA: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 10

Genetic Interactions
  • Negative Genetic: 109
  • Positive Genetic: 8
  • Synthetic Growth Defect: 45
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 223
Molecular Weight (Da) 25,268
Isoelectric Point (pI) 5.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:609753 to 609082 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..672 609753..609082 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000397
References cited on this page View Complete Literature Guide for MED8
1) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9