MED7/YOL135C Summary Help

Standard Name MED7 1
Systematic Name YOL135C
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 2)
Name Description MEDiator complex 1
Chromosomal Location
ChrXV:70044 to 69376 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MED7 GO evidence and references
  View Computational GO annotations for MED7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
null
reduction of function
repressible
Resources
323 total interaction(s) for 160 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 98
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 44
  • Co-crystal Structure: 3
  • Co-fractionation: 1
  • Co-purification: 23
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 45
  • Positive Genetic: 3
  • Synthetic Growth Defect: 72
  • Synthetic Lethality: 5
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 222
Molecular Weight (Da) 25,585
Isoelectric Point (pI) 6.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:70044 to 69376 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..669 70044..69376 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005495
References cited on this page View Complete Literature Guide for MED7
1) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9