MED6/YHR058C Summary Help

Standard Name MED6 1
Systematic Name YHR058C
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5)
Name Description MEDiator complex 1
Chromosomal Location
ChrVIII:219884 to 218997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MED6 GO evidence and references
  View Computational GO annotations for MED6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 150 genes
Resources
Classical genetics
conditional
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
125 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 28
  • Biochemical Activity: 1
  • Co-purification: 17
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 5

Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 295
Molecular Weight (Da) 32,819
Isoelectric Point (pI) 5.05
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:219884 to 218997 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..888 219884..218997 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001100
References cited on this page View Complete Literature Guide for MED6
1) Lee YC, et al.  (1997) A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans. Mol Cell Biol 17(8):4622-32
2) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
3) Guglielmi B, et al.  (2004) A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32(18):5379-91
4) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76