MED2/YDL005C Summary Help

Standard Name MED2 1
Systematic Name YDL005C
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; relocalizes to the cytosol in response to hypoxia (1, 2, 3)
Name Description MEDiator complex 1
Chromosomal Location
ChrIV:442309 to 441014 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MED2 GO evidence and references
  View Computational GO annotations for MED2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 980 genes
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
217 total interaction(s) for 132 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 65
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 31
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • Co-purification: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 57
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Positive Genetic: 16
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 431
Molecular Weight (Da) 47,717
Isoelectric Point (pI) 4.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:442309 to 441014 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1296 442309..441014 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002163
References cited on this page View Complete Literature Guide for MED2
1) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
2) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
3) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30