MDY2/YOL111C Summary Help

Standard Name MDY2 1
Systematic Name YOL111C
Alias TMA24 2 , GET5 3
Feature Type ORF, Verified
Description Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes (1, 2, 3, 4)
Name Description Mating-Deficient Yeast 1, 2
Chromosomal Location
ChrXV:108896 to 108258 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MDY2 GO evidence and references
  View Computational GO annotations for MDY2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
173 total interaction(s) for 80 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 16
  • Co-crystal Structure: 4
  • Co-purification: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 11

Genetic Interactions
  • Negative Genetic: 29
  • Phenotypic Enhancement: 53
  • Phenotypic Suppression: 16
  • Positive Genetic: 5
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 212
Molecular Weight (Da) 23,732
Isoelectric Point (pI) 10.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:108896 to 108258 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..639 108896..108258 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005471
References cited on this page View Complete Literature Guide for MDY2
1) Hu Z, et al.  (2006) Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae. J Cell Sci 119(Pt 2):326-38
2) Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
3) Jonikas MC, et al.  (2009) Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum. Science 323(5922):1693-7
4) Chang YW, et al.  (2010) Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1. J Biol Chem 285(13):9962-70