MDN1/YLR106C Summary Help

Standard Name MDN1 1
Systematic Name YLR106C
Alias REA1
Feature Type ORF, Verified
Description Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus (1, 2, 3, 4)
Name Description MiDasiN 1
Chromosomal Location
ChrXII:363738 to 349006 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MDN1 GO evidence and references
  View Computational GO annotations for MDN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Large-scale survey
null
reduction of function
repressible
Resources
111 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 87
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 1
  • Co-purification: 2
  • Two-hybrid: 11

Genetic Interactions
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 4,910
Molecular Weight (Da) 559,302
Isoelectric Point (pI) 4.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:363738 to 349006 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..14733 363738..349006 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004096
References cited on this page View Complete Literature Guide for MDN1
1) Garbarino J and Gibbons I  (2002) Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein. BMC Genomics 3(1):18
2) Nissan TA, et al.  (2004) A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol Cell 15(2):295-301
3) Ulbrich C, et al.  (2009) Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits. Cell 138(5):911-22
4) Bassler J, et al.  (2010) The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly. Mol Cell 38(5):712-21