MDL2/YPL270W Summary Help

Standard Name MDL2 1
Systematic Name YPL270W
Feature Type ORF, Verified
Description Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis (1, 2, 3 and see Summary Paragraph)
Name Description MultiDrug resistance-Like 1
Chromosomal Location
ChrXVI:30482 to 32803 | ORF Map | GBrowse
Gene Ontology Annotations All MDL2 GO evidence and references
  View Computational GO annotations for MDL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
93 total interaction(s) for 72 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • PCA: 2

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 1
  • Negative Genetic: 65
  • Phenotypic Enhancement: 1
  • Positive Genetic: 10
  • Synthetic Growth Defect: 5
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 773
Molecular Weight (Da) 85,136
Isoelectric Point (pI) 9.75
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:30482 to 32803 | ORF Map | GBrowse
Last Update Coordinates: 2003-09-22 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2322 30482..32803 2003-09-22 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000006191

MDL1 and MDL2 encode members of the ATP-binding Cassette (ABC) transporter family that reside in the mitochondrial inner membrane (1, 2). They are classified as "half-molecule" family members because they are comprised of one transmembrane domain and one nucleotide-binding domain, in contrast to "full-size" family members in which this arrangement is repeated in tandem. Mdl1p and Mdl2p are similar to each other and to other ABC family members, in particular to human TAP1 and TAP2, which form a heterodimer that transports peptides from the cytoplasm into the endoplasmic reticulum (1). Defects in TAP1 and TAP2 are linked to bare lymphocyte syndrome (OMIM) and Wegener-like granulomatosis (OMIM).

Although Mdl2p is very similar to Mdl1p, which exports peptides from mitochondria, the mdl2 null mutation does not affect peptide export. In contrast to mdl1, the mdl null mutation prevents respiratory growth at high temperature (2). Large-scale studies have also detected osmotic stress resistance and oleate sensitivity phenotypes of the mdl2 null mutant (4, 5).

Last updated: 2010-02-09 Contact SGD

References cited on this page View Complete Literature Guide for MDL2
1) Dean M, et al.  (1994) Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily. Yeast 10(3):377-83
2) Young L, et al.  (2001) Role of the ABC transporter Mdl1 in peptide export from mitochondria. Science 291(5511):2135-8
3) Breker M, et al.  (2013) A novel single-cell screening platform reveals proteome plasticity during yeast stress responses. J Cell Biol 200(6):839-50
4) Yoshikawa K, et al.  (2009) Comprehensive phenotypic analysis for identification of genes affecting growth under ethanol stress in Saccharomyces cerevisiae. FEMS Yeast Res 9(1):32-44
5) Lockshon D, et al.  (2007) The sensitivity of yeast mutants to oleic Acid implicates the peroxisome and other processes in membrane function. Genetics 175(1):77-91