MCM10/YIL150C Summary Help

Standard Name MCM10 1
Systematic Name YIL150C
Alias DNA43 2
Feature Type ORF, Verified
Description Essential chromatin-associated protein; involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins; required to stabilize the catalytic subunit of DNA polymerase-alpha (1, 3, 4 and see Summary Paragraph)
Name Description MiniChromosome Maintenance 5
Chromosomal Location
ChrIX:62728 to 61013 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MCM10 GO evidence and references
  View Computational GO annotations for MCM10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
overexpression
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
103 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 16
  • Co-localization: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 23

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 23
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 12
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 571
Molecular Weight (Da) 65,815
Isoelectric Point (pI) 10.46
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:62728 to 61013 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1716 62728..61013 1994-12-10 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001412
SUMMARY PARAGRAPH for MCM10

DNA43, also known as MCM10, is an essential gene that was independently identified in a screen for temperature sensitive mutants (6) and in a screen for mutations that cause defects in the stability of centromeric plasmids (minichromosome maintenance defects, Mcm-) (1, 7). The phenotypes of dna43 mutants strongly suggest that Dna43p plays a role in the initiation of DNA replication. The temperature sensitive dna43-1 allele causes cells to arrest with large buds and unreplicated DNA at the restrictive temperature (6). In addition to its Mcm- defect, the mcm10-1 allele also reduces the frequency of DNA replication initiation at chromosomal origins, and causes DNA replication forks to pause during elongation at origins that have not initiated (1). The pausing depends on the integrity of sequences essential for origin function that are occupied by the pre-replicative complex (pre-RC) prior to initiation (3, 7). The pre-RC is an assembly of proteins that forms at origins of DNA replication between late M phase and the G1/S transition, and includes proteins believed to act in DNA replication initiation (8, 9, 10, 11, 12). Dna43p interacts physically and genetically with members of the Mcm2-7 family, which are pre-RC components required for the initiation of DNA replication (1, 3, 7). Dna43p is associated with chromatin throughout the cell cycle, and its presence is required for Mcm2p, but not the origin recognition complex subunit Orc3p, to bind chromatin in G1 (3). The S. pombe Cdc23 protein is 22% identical to Dna43p and can complement the dna43-1 mutation (13).

Last updated: 2000-05-10 Contact SGD

References cited on this page View Complete Literature Guide for MCM10
1) Merchant AM, et al.  (1997) A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae. Mol Cell Biol 17(6):3261-71
2) Dumas LB, et al.  (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol Gen Genet 187(1):42-6
3) Homesley L, et al.  (2000) Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev 14(8):913-26
4) Ricke RM and Bielinsky AK  (2006) A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast. J Biol Chem 281(27):18414-25
5) Maine GT, et al.  (1984) Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 106(3):365-85
6) Solomon NA, et al.  (1992) Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae. Yeast 8(4):273-89
7) Tye BK  (1999) MCM proteins in DNA replication. Annu Rev Biochem 68:649-86
8) Leatherwood J  (1998) Emerging mechanisms of eukaryotic DNA replication initiation. Curr Opin Cell Biol 10(6):742-8
9) Dutta A and Bell SP  (1997) Initiation of DNA replication in eukaryotic cells. Annu Rev Cell Dev Biol 13():293-332
10) Santocanale C and Diffley JF  (1996) ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J 15(23):6671-9
11) Cocker JH, et al.  (1996) An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature 379(6561):180-2
12) Diffley JF, et al.  (1994) Two steps in the assembly of complexes at yeast replication origins in vivo. Cell 78(2):303-16
13) Aves SJ, et al.  (1998) The essential schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene. Curr Genet 34(3):164-71