MCK1/YNL307C Summary Help

Standard Name MCK1 1
Systematic Name YNL307C
Alias YPK1 2 , CMS1 1 , 3
Feature Type ORF, Verified
Description Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication (1, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description Meiotic and Centromere regulatory ser, tyr-Kinase
Chromosomal Location
ChrXIV:57573 to 56446 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MCK1 GO evidence and references
  View Computational GO annotations for MCK1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
607 total interaction(s) for 426 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 114
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 38
  • Dosage Lethality: 3
  • Dosage Rescue: 16
  • Negative Genetic: 270
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 1
  • Positive Genetic: 35
  • Synthetic Growth Defect: 26
  • Synthetic Lethality: 16
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 375
Molecular Weight (Da) 43,136
Isoelectric Point (pI) 8.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:57573 to 56446 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1128 57573..56446 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005251
SUMMARY PARAGRAPH for MCK1

MCK1 encodes a dual-specificity protein kinase related to mammalian glycogen synthase kinases in the GSK-3 family (4, 11). Mammalian GSK-3 kinases are involved in signal transduction and in regulating glycogen metabolism, gene expression, development, and cell differentiation (reviewed in references 12 and 13).

The phenotypes of mck1 mutants and of overexpressing wild type MCK1 suggest that Mck1p plays roles in chromosome segregation and in regulating entry into meiosis (5, 1). Cells lacking MCK1 are viable but cold sensitive, and show increased mitotic chromosome loss at semipermissive temperatures or in the presence of the microtubule destabilizing drug benomyl (1). MCK1 shows genetic interactions with the centromeric sequence element CDEIII, and with CBF2 and CBF5, which encode centromere binding proteins (1, 14). Mck1p phosphorylates Cbf2p (14) and interacts physically with Cbf2p and Cbf5p in vivo (15).

In diploids, mck1 mutations cause delayed and decreased levels of sporulation and defects in ascus formation (5). MCK1 interacts genetically with IME1, which encodes a sporulation-specific transcriptional activator (5, 16). The protein phosphatase Yvh1p may act upstream of Mck1p in regulating sporulation (17). Mck1p autophosphorylates on tyrosine and serine residues (4); the tyrosine phosphorylation is elevated during sporulation in strains lacking the protein tyrosine phosphatases Ptp2p and Ptp3p (18).

Mck1p may also be involved in regulating intermediary metabolism; MCK1 interacts genetically with CDC19, which encodes pyruvate kinase (19). Purified Mck1p interacts physically with pyruvate kinase in vitro, and may regulate pyruvate kinase activity by direct phosphorylation (19).

Three other protein kinases of the GSK-3 family have been identified in yeast; they are encoded by RIM11/MDS1, YGK3, and MRK1 (20, 11, 21, 22). Related kinases have also been found in Drosophila, Arabidopsis, and Kluyveromyces lactis as well as mammals (11, 23, 24, 12, 13).
Note: MCK1, YPK1/YKL126W, which encodes a ser/thr protein kinase and TOK1/YJL093C, which encodes a potassium channel have been refered to as YPK1 in the literature.

Last updated: 2000-10-02 Contact SGD

References cited on this page View Complete Literature Guide for MCK1
1) Shero JH and Hieter P  (1991) A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1). Genes Dev 5(4):549-60
2) Dailey D, et al.  (1990) Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity. Mol Cell Biol 10(12):6244-56
3) Schero, J.  (1992) Personal Communication, Mortimer Map Edition 11
4) Lim MY, et al.  (1993) Yeast MCK1 protein kinase autophosphorylates at tyrosine and serine but phosphorylates exogenous substrates at serine and threonine. J Biol Chem 268(28):21155-64
5) Neigeborn L and Mitchell AP  (1991) The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Genes Dev 5(4):533-48
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Kishi T, et al.  (2007) The SCFCdc4 ubiquitin ligase regulates calcineurin signaling through degradation of phosphorylated Rcn1, an inhibitor of calcineurin. Proc Natl Acad Sci U S A 104(44):17418-23
8) McQueen J, et al.  (2012) The Mck1 GSK-3 kinase inhibits the activity of Clb2-Cdk1 post-nuclear division. Cell Cycle 11(18):3421-32
9) Ikui AE, et al.  (2012) A Yeast GSK-3 Kinase Mck1 Promotes Cdc6 Degradation to Inhibit DNA Re-Replication. PLoS Genet 8(12):e1003099
10) Moir RD and Willis IM  (2013) Regulation of pol III transcription by nutrient and stress signaling pathways. Biochim Biophys Acta 1829(3-4):361-75
11) Bianchi MW, et al.  (1993) A Saccharomyces cerevisiae protein-serine kinase related to mammalian glycogen synthase kinase-3 and the Drosophila melanogaster gene shaggy product. Gene 134(1):51-6
12) Woodgett JR  (1994) Regulation and functions of the glycogen synthase kinase-3 subfamily. Semin Cancer Biol 5(4):269-75
13) Dale TC  (1998) Signal transduction by the Wnt family of ligands. Biochem J 329 ( Pt 2)():209-23
14) Jiang W, et al.  (1995) Overexpression of the yeast MCK1 protein kinase suppresses conditional mutations in centromere-binding protein genes CBF2 and CBF5. Mol Gen Genet 246(3):360-6
15) Jiang W and Koltin Y  (1996) Two-hybrid interaction of a human UBC9 homolog with centromere proteins of Saccharomyces cerevisiae. Mol Gen Genet 251(2):153-60
16) Mitchell AP  (1994) Control of meiotic gene expression in Saccharomyces cerevisiae. Microbiol Rev 58(1):56-70
17) Beeser AE and Cooper TG  (1999) The dual-specificity protein phosphatase Yvh1p acts upstream of the protein kinase mck1p in promoting spore development in Saccharomyces cerevisiae. J Bacteriol 181(17):5219-24
18) Zhan XL, et al.  (2000) Essential functions of protein tyrosine phosphatases PTP2 and PTP3 and RIM11 tyrosine phosphorylation in Saccharomyces cerevisiae meiosis and sporulation. Mol Biol Cell 11(2):663-76
19) Brazill DT, et al.  (1997) Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. J Bacteriol 179(13):4415-8
20) Puziss JW, et al.  (1994) MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase 3. Mol Cell Biol 14(1):831-9
21) Hardy TA, et al.  (1995) Novel Saccharomyces cerevisiae gene, MRK1, encoding a putative protein kinase with similarity to mammalian glycogen synthase kinase-3 and Drosophila Zeste-White3/Shaggy. Biochem Biophys Res Commun 208(2):728-34
22) Hirata Y, et al.  (2003) Yeast glycogen synthase kinase-3 activates Msn2p-dependent transcription of stress responsive genes. Mol Biol Cell 14(1):302-12
23) Piao HL, et al.  (1999) An Arabidopsis GSK3/shaggy-like gene that complements yeast salt stress-sensitive mutants is induced by NaCl and abscisic acid. Plant Physiol 119(4):1527-34
24) Rodriguez-Belmonte E, et al.  (1998) The Kluyveromyces lactis gene KLGSK-3 combines functions which in Saccharomyces cerevisiae are performed by MCK1 and MSD1. Curr Genet 33(4):262-7