MBF1/YOR298C-A Summary Help

Standard Name MBF1 1
Systematic Name YOR298C-A
Alias SUF13
Feature Type ORF, Verified
Description Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description Multiprotein Bridging Factor 1
Chromosomal Location
ChrXV:877685 to 877230 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 165 cM
Gene Ontology Annotations All MBF1 GO evidence and references
  View Computational GO annotations for MBF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 14 genes
Regulators 24 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
80 total interaction(s) for 70 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • PCA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 46
  • Phenotypic Enhancement: 2
  • Positive Genetic: 6
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 151
Molecular Weight (Da) 16,404
Isoelectric Point (pI) 11.35
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:877685 to 877230 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 165 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2004-02-12
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..456 877685..877230 2011-02-03 2004-02-12
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007253
References cited on this page View Complete Literature Guide for MBF1
1) Takemaru Ki, et al.  (1997) Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein. Proc Natl Acad Sci U S A 94(14):7251-6
2) Culbertson MR, et al.  (1982) Frameshift suppression in Saccharomyces cerevisiae. V. Isolation and genetic properties of nongroup-specific suppressors. Genetics 102(3):361-78
3) Takemaru K, et al.  (1998) Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation. Mol Cell Biol 18(9):4971-6
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76