Standard Name	MAK5 1
Systematic Name	YBR142W
Feature Type	ORF, Verified
Description	Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits (1, 2)
Name Description	MAintenance of Killer 3

Chromosomal Location	ChrII:528317 to 530638 | ORF Map | GBrowse
	Genetic position: 80 cM

	View Computational GO annotations for MAK5
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (TAS)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) ribosomal large subunit biogenesis (IMP)
Cellular Component
Manually curated	nucleolus (IDA)

Classical genetics
dominant negative	vegetative growth: decreased
null	dessication resistance: increased inviable
Large-scale survey
conditional	heat sensitivity: increased
null	inviable resistance to 5-fluorouracil: decreased
overexpression	vegetative growth: decreased rate vegetative growth: decreased
reduction of function	competitive fitness: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	125 total interaction(s) for 84 unique genes/features.
Physical Interactions	Affinity Capture-MS: 104 Affinity Capture-RNA: 2 Affinity Capture-Western: 6 Biochemical Activity: 2
Genetic Interactions	Negative Genetic: 3 Phenotypic Suppression: 4 Positive Genetic: 2 Synthetic Lethality: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:528317 to 530638 | ORF Map | GBrowse
	Genetic position: 80 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2322 528317..530638 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000346