MAK31/YCR020C-A Summary

Standard Name	MAK31
Systematic Name	YCR020C-A
Alias	LSM9 , SMX1 , NAA38 1
Feature Type	ORF, Verified
Description	Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family (2, 3 and see Summary Paragraph)
Name Description	MAintenance of Killer 4

Chromosomal Location	ChrIII:155096 to 154830 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All MAK31 GO evidence and references


Molecular Function
Manually curated	contributes_to peptide alpha-N-acetyltransferase activity (IMP)
Biological Process
Manually curated	N-terminal protein amino acid acetylation (IMP)
Cellular Component
Manually curated	NatC complex (IDA)

Mutant phenotypes All MAK31 Phenotype evidence and references

Classical genetics
null	LA-dsRNA accumulation: absent Imh1p-GFP distribution: abnormal Trm1p-II-GFP distribution: abnormal
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased resistance to arsenite(3-): decreased resistance to L-canavanine: increased telomere length: increased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MAK31 Interaction evidence and references

	53 total interaction(s) for 44 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 3 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 27 Positive Genetic: 3 Synthetic Growth Defect: 3 Synthetic Lethality: 2 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MAK31 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIII:155096 to 154830 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1997-01-28

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..267	155096..154830	2011-02-03	1997-01-28

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000614

SUMMARY PARAGRAPH for MAK31

Although MAK31/LSM9 has been reported to contain an Sm domain (5), it is slightly more divergent than the other LSM proteins (Lsm1p, Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, Lsm7p, and Lsm8p) and has not been shown to interact with any of the snRNAs (5, 3). Mak31p is also known to be a required subunit of the NatC acetyltransferase complex (3).

Last updated: 2006-01-26

References cited on this page View Complete Literature Guide for MAK31

1)	Polevoda B, et al. (2009) A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates. BMC Proc 3 Suppl 6:S2
2)	Wickner RB and Leibowitz MJ (1976) Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J Mol Biol 105(3):427-43
3)	Polevoda B and Sherman F (2001) NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p. J Biol Chem 276(23):20154-9
4)	Wickner RB (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32
5)	Salgado-Garrido J, et al. (1999) Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin. EMBO J 18(12):3451-62