MAK16/YAL025C Summary Help

Standard Name MAK16 1
Systematic Name YAL025C
Feature Type ORF, Verified
Description Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus (2, 3, 4)
Name Description MAintenance of Killer 5
Chromosomal Location
ChrI:101145 to 100225 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -31 cM
Gene Ontology Annotations All MAK16 GO evidence and references
  View Computational GO annotations for MAK16
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
Resources
62 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 38
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 306
Molecular Weight (Da) 35,695
Isoelectric Point (pI) 4.9
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:101145 to 100225 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -31 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..921 101145..100225 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000023
References cited on this page View Complete Literature Guide for MAK16
1) Toh-E A and Wickner RB  (1980) "Superkiller" mutations suppress chromosomal mutations affecting double-stranded RNA killer plasmid replication in saccharomyces cerevisiae. Proc Natl Acad Sci U S A 77(1):527-30
2) Ohtake Y and Wickner RB  (1995) Yeast virus propagation depends critically on free 60S ribosomal subunit concentration. Mol Cell Biol 15(5):2772-81
3) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4) Pellett S and Tracy JW  (2006) Mak16p is required for the maturation of 25S and 5.8S rRNAs in the yeast Saccharomyces cerevisiae. Yeast 23(7):495-506
5) Wickner RB  (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32