MAG1/YER142C Summary Help

Standard Name MAG1 1
Systematic Name YER142C
Alias MMS5 2
Feature Type ORF, Verified
Description 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress (3, 4)
Name Description 3-MethylAdenine DNA Glycosylase 3
Chromosomal Location
ChrV:456036 to 455146 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 110 cM
Gene Ontology Annotations All MAG1 GO evidence and references
  View Computational GO annotations for MAG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
89 total interaction(s) for 72 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Biochemical Activity: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 27
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 6
  • Positive Genetic: 5
  • Synthetic Growth Defect: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 296
Molecular Weight (Da) 34,333
Isoelectric Point (pI) 8.16
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:456036 to 455146 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 110 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..891 456036..455146 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000944
References cited on this page View Complete Literature Guide for MAG1
1) Xiao W, et al.  (1994) The MAG1* 3-methyladenine DNA glycosylase gene is closely linked to the SPT15 TATA-binding TFIID gene on chromosome V-R in Saccharomyces cerevisiae. Yeast 10(5):687-91
2) Prakash L and Prakash S  (1977) Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics 86(1):33-55
3) Chen J, et al.  (1989) Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli. Proc Natl Acad Sci U S A 86(20):7961-5
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76