LYS9/YNR050C Summary 
LYS9 BASIC INFORMATION
| Standard Name | LYS9 |
|---|---|
| Systematic Name | YNR050C |
| Alias | LYS13 |
| Feature Type | ORF, Verified |
| Description | Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 (1, 2 and see Summary Paragraph) 
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| Name Description | LYSine requiring |
| GO Annotations | All LYS9 GO evidence and references |
|---|---|
| View Computational GO annotations for LYS9 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| High-throughput |
| Pathways |
|---|
| Mutant Phenotype | All LYS9 Phenotype details and references |
|---|---|
| Classical genetics | |
| overexpression | |
| Large-scale survey | |
| null |
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| Interactions | LYS9 All interactions details and references |
|---|---|
| 17 total interaction(s) for 14 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000005333 |
|---|
LYS9 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for LYS9
SUMMARY PARAGRAPH for LYS9
About lysine biosynthesis
S. cerevisiae synthesizes the essential amino acid L-lysine via the L-alpha-aminoadipic acid pathway instead of the diaminopmelate pathway (3). Originally proposed to be characteristic of fungi, recent studies suggest prokaryotes also synthesize lysine via the alpha-aminoadipic acid pathway (4). Intermediates in this pathway are often incorporated into secondary metabolites. For example, it has been well- studied that alpha-aminoadipate is required for penicillin production (3). Regulation of the lysine biosynthetic pathway in S. cerevisiae is an interaction between general amino acid control (via Gcn4p) (5), feedback inhibition of homocitrate synthase activity by lysine (6), and induction of Lys14p by alpha-aminoadipate semialdehyde (7).
Last updated: 2007-10-04
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for LYS9]
| 1) | Storts DR and Bhattacharjee JK (1987) Purification and properties of saccharopine dehydrogenase (glutamate forming) in the Saccharomyces cerevisiae lysine biosynthetic pathway. J Bacteriol 169(1):416-8 |
| 2) | Studte P, et al. (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77 |
| 3) | Zabriskie TM and Jackson MD (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17(1):85-97 |
| 4) | Nishida H and Nishiyama M (2000) What is characteristic of fungal lysine synthesis through the alpha-aminoadipate pathway? J Mol Evol 51(3):299-302 |
| 5) | Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press |
| 6) | Feller A, et al. (1999) In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur J Biochem 261(1):163-70 |
| 7) | El Alami M, et al. (2000) Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae. Curr Genet 38(2):78-86 |
