LYS14/YDR034C Summary Help

Standard Name LYS14 1
Systematic Name YDR034C
Feature Type ORF, Verified
Description Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer (2, 3, 4, 5)
Name Description LYSine requiring 1
Chromosomal Location
ChrIV:512109 to 509737 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 8 cM
Gene Ontology Annotations All LYS14 GO evidence and references
  View Computational GO annotations for LYS14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
19 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 11
  • Synthetic Growth Defect: 2

Expression Summary
Length (a.a.) 790
Molecular Weight (Da) 89,396
Isoelectric Point (pI) 6.92
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:512109 to 509737 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 8 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2373 512109..509737 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002441
References cited on this page View Complete Literature Guide for LYS14
1) Borell CW, et al.  (1984) Two unlinked lysine genes (LYS9 and LYS14) are required for the synthesis of saccharopine reductase in Saccharomyces cerevisiae. J Bacteriol 159(1):429-32
2) Becker B, et al.  (1998) A nonameric core sequence is required upstream of the LYS genes of Saccharomyces cerevisiae for Lys14p-mediated activation and apparent repression by lysine. Mol Microbiol 29(1):151-63
3) Feller A, et al.  (1994) Repression of the genes for lysine biosynthesis in Saccharomyces cerevisiae is caused by limitation of Lys14-dependent transcriptional activation. Mol Cell Biol 14(10):6411-8
4) Ramos F, et al.  (1988) Control of enzyme synthesis in the lysine biosynthetic pathway of Saccharomyces cerevisiae. Evidence for a regulatory role of gene LYS14. Eur J Biochem 171(1-2):171-6
5) Alami ME, et al.  (2002) The proper folding of a long C-terminal segment of the yeast Lys14p regulator is required for activation of LYS genes in response to the metabolic effector. Mol Microbiol 43(6):1629-39
6) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
7) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87