LTE1/YAL024C Summary Help

Standard Name LTE1 1
Systematic Name YAL024C
Alias MSI2
Feature Type ORF, Verified
Description Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures (2, 3 and see Summary Paragraph)
Name Description Low Temperature Essential 4
Chromosomal Location
ChrI:105872 to 101565 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -31 cM
Gene Ontology Annotations All LTE1 GO evidence and references
  View Computational GO annotations for LTE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
592 total interaction(s) for 391 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 72
  • Affinity Capture-Western: 11
  • Biochemical Activity: 3
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Lethality: 3
  • Dosage Rescue: 23
  • Negative Genetic: 89
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Positive Genetic: 2
  • Synthetic Growth Defect: 236
  • Synthetic Lethality: 110
  • Synthetic Rescue: 27

Expression Summary
Length (a.a.) 1,435
Molecular Weight (Da) 163,149
Isoelectric Point (pI) 6.43
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:105872 to 101565 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -31 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4308 105872..101565 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000022

LTE1 is essential for termination of M phase at low temperatures, along with TEM1, and CDC15 (5), all part of the mitotic exit network which is an elaborate signaling system comprising at least eight essential genes: CDC5, CDC14, CDC15, DBF2, DBF20, LTE1, MOB1, and TEM1 (6, 7). LTE1 was originally discovered as a locus essential for growth at 8 C, and was named for this trait as Low Temperature Essential 1 (4). It was later inferred from sequence similarities that LTE1 must code for a guanine nucleotide exchange factor (8). Genetic evidence suggests that Lte1p activates the GTPase Tem1p which activates the protein kinase Cdc15p. Cdc15p then relieves the inhibition of the protein phosphatase Cdc14p by Net1p, thereby allowing exit from mitosis (7). In a normal cell cycle, Bub2p bound to Tem1p restrains mitotic exit until the daughter-bound centrosome (with which both Bub2p and Tem1p preferentially associate) comes into contact with Lte1p in the bud, effectively coupling mitotic exit with nuclear segregation (9). Some evidence suggests that the spatial separation of Tem1p and Lte1p is a key mechanism for controlling the timing of mitotic exit (9).

Last updated: 2002-10-17 Contact SGD

References cited on this page View Complete Literature Guide for LTE1
1) Kaback, D.  (1989) Personal Communication, Mortimer Map Edition 10
2) Shirayama M, et al.  (1994) Isolation of a CDC25 family gene, MSI2/LTE1, as a multicopy suppressor of ira1. Yeast 10(4):451-61
3) Geymonat M, et al.  (2009) Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1. J Cell Biol 187(4):497-511
4) Wickner RB, et al.  (1987) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the MAK16 gene and analysis of an adjacent gene essential for growth at low temperatures. Yeast 3(1):51-7
5) Shirayama M, et al.  (1994) The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase. Mol Cell Biol 14(11):7476-82
6) Menssen R, et al.  (2001) Asymmetric spindle pole localization of yeast Cdc15 kinase links mitotic exit and cytokinesis. Curr Biol 11(5):345-50
7) Shou W, et al.  (1999) Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97(2):233-44
8) Keng T, et al.  (1994) LTE1 of Saccharomyces cerevisiae is a 1435 codon open reading frame that has sequence similarities to guanine nucleotide releasing factors. Yeast 10(7):953-8
9) Schuyler SC and Pellman D  (2001) Search, capture and signal: games microtubules and centrosomes play. J Cell Sci 114(Pt 2):247-55