LSB1/YGR136W Summary Help

Standard Name LSB1 1
Systematic Name YGR136W
Feature Type ORF, Verified
Description Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description Las Seventeen Binding protein 1
Chromosomal Location
ChrVII:762428 to 763153 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LSB1 GO evidence and references
  View Computational GO annotations for LSB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 83 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
Resources
54 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-Luminescence: 1
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • Biochemical Activity: 1
  • Co-localization: 1
  • Protein-peptide: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 4
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 241
Molecular Weight (Da) 26,139
Isoelectric Point (pI) 4.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:762428 to 763153 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..726 762428..763153 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003368
References cited on this page View Complete Literature Guide for LSB1
1) Madania A, et al.  (1999) The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex. Mol Biol Cell 10(10):3521-38
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Spiess M, et al.  (2013) Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis. PLoS One 8(4):e61147