LPD1/YFL018C Summary Help

LPD1 BASIC INFORMATION

Standard Name LPD1
Systematic Name YFL018C
Alias HPD1
Feature Type ORF, Verified
Description Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes (1 and see Summary Paragraph)
Name Description LiPoamide Dehydrogenase 2
GO Annotations All LPD1 GO evidence and references
    View Computational GO annotations for LPD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant Phenotype All LPD1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions LPD1 All interactions details and references
30 total interaction(s) for 16 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 1
  • Two-hybrid: 1
Genetic Interactions
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
Sequence Information
ChrVI:103121 to 101622 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1500 103121..101622 1996-07-31 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001876

LPD1 RESOURCES

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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for LPD1

SUMMARY PARAGRAPH for LPD1

LPD1 encodes dihydrolipoamide dehydrogenase, which is found in the mitochondrial matrix (3, 1) and is an essential enzyme required for the glycine cleavage system, glycolysis and TCA cycle. Lpd1p is a component of the glycine decarboxylase complex (GDC), 2-oxoglutarate dehydrogenase (OGDH), and pyruvate dehydrogenase (PDH) complexes (3, 2). Mutations in LPD1 abolish activity of the glycine decarboxylase, 2-oxoglutarate dehydrogenase, and pyruvate dehydrogenase complexes (4, 5, 6). GDC is a multienzyme complex that catalyzes the reversible oxidative cleavage of glycine into CO2 and NH3 and connects the metabolism of one, two and three-carbon compounds as shown in this pathway diagram (7). PDH catalyses the conversion of pyruvate to acetyl-CoA while OGDH catalyses the oxidative decarboxylation of 2-oxoglutarate to succinyl-CoA, which is part of the TCA cycle (3). In order to bind to the E2 (Lat1p) core of the pyruvate dehydrogenase complex, Lpd1p (E3) requires the activity of Pdx1p (Lpd1p-binding protein; Protein X) (8).

LPD1 is subject to catabolite repression and transcription of LPD1 for release from glucose repression is activated by the HAP2/HAP3/HAP4 transcription factor complex (9). In addition, LPD1 is also regulated by the Gcn4p transcription factor under conditions of amino acid starvation (6).

Lpd1p has strong sequence similarity to dihydrolipoamide dehydrogenases in prokaryotes and mammals (1, 10). Mutations in DLD, the human dihydrolipoamide dehydrogenase gene, are responsible for some cases of lactic acidosis (maple syrup urine disease, type III) (OMIM) (11).

Last updated: 2008-04-03

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for LPD1]

1) Ross J, et al.  (1988) The nucleotide sequence of the LPD1 gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae: comparison between eukaryotic and prokaryotic sequences for related enzymes and identification of potential upstream control sites. J Gen Microbiol 134(5):1131-9
2) Roy DJ and Dawes IW  (1987) Cloning and characterization of the gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae. J Gen Microbiol 133(4):925-33
3) Pronk JT, et al.  (1996) Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12(16):1607-33
4) Sinclair DA and Dawes IW  (1995) Genetics of the synthesis of serine from glycine and the utilization of glycine as sole nitrogen source by Saccharomyces cerevisiae. Genetics 140(4):1213-22
5) Dickinson JR, et al.  (1997) A 13C nuclear magnetic resonance investigation of the metabolism of leucine to isoamyl alcohol in Saccharomyces cerevisiae. J Biol Chem 272(43):26871-8
6) Zaman Z, et al.  (1999) Transcription factor GCN4 for control of amino acid biosynthesis also regulates the expression of the gene for lipoamide dehydrogenase. Biochem J 340 ( Pt 3)():855-62
7) Gelling CL, et al.  (2004) Identification of a novel one-carbon metabolism regulon in Saccharomyces cerevisiae. J Biol Chem 279(8):7072-81
8) Lawson JE, et al.  (1991) Disruption and mutagenesis of the Saccharomyces cerevisiae PDX1 gene encoding the protein X component of the pyruvate dehydrogenase complex. Biochemistry 30(11):2834-9
9) Bowman SB, et al.  (1992) Positive regulation of the LPD1 gene of Saccharomyces cerevisiae by the HAP2/HAP3/HAP4 activation system. Mol Gen Genet 231(2):296-303
10) Browning KS, et al.  (1988) Nucleotide sequence for yeast dihydrolipoamide dehydrogenase. Proc Natl Acad Sci U S A 85(6):1831-4
11) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10