LIA1/YJR070C Summary Help

Standard Name LIA1 1
Systematic Name YJR070C
Alias MMD1 2
Feature Type ORF, Verified
Description Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description Ligand of eIF5A 1
Chromosomal Location
ChrX:570598 to 569621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All LIA1 GO evidence and references
  View Computational GO annotations for LIA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
unspecified
Resources
74 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • PCA: 3
  • Reconstituted Complex: 7
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 27
  • Positive Genetic: 4
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 325
Molecular Weight (Da) 36,164
Isoelectric Point (pI) 4.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:570598 to 569621 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..978 570598..569621 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003831
References cited on this page View Complete Literature Guide for LIA1
1) Thompson GM, et al.  (2003) Mapping eIF5A binding sites for Dys1 and Lia1: in vivo evidence for regulation of eIF5A hypusination. FEBS Lett 555(3):464-8
2) Weir BA and Yaffe MP  (2004) Mmd1p, a novel, conserved protein essential for normal mitochondrial morphology and distribution in the fission yeast Schizosaccharomyces pombe. Mol Biol Cell 15(4):1656-65
3) Park JH, et al.  (2006) Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme. Proc Natl Acad Sci U S A 103(1):51-6
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76