LEO1/YOR123C Summary Help

Standard Name LEO1 1
Systematic Name YOR123C
Feature Type ORF, Verified
Description Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay (2, 3, 4, 5, 6, 7)
Name Description LEft Open reading frame 1
Chromosomal Location
ChrXV:554570 to 553176 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All LEO1 GO evidence and references
  View Computational GO annotations for LEO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
527 total interaction(s) for 274 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 71
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 9
  • Biochemical Activity: 9
  • Co-fractionation: 1
  • Protein-RNA: 4
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 280
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 7
  • Positive Genetic: 47
  • Synthetic Growth Defect: 55
  • Synthetic Lethality: 20
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 464
Molecular Weight (Da) 53,920
Isoelectric Point (pI) 4.15
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:554570 to 553176 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1395 554570..553176 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005649
References cited on this page View Complete Literature Guide for LEO1
1) Magdolen V, et al.  (1994) The gene LEO1 on yeast chromosome XV encodes a non-essential, extremely hydrophilic protein. Biochim Biophys Acta 1218(2):205-9
2) Mueller CL and Jaehning JA  (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
3) Betz JL, et al.  (2002) Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism. Mol Genet Genomics 268(2):272-85
4) Krogan NJ, et al.  (2003) The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell 11(3):721-9
5) Wood A, et al.  (2003) The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. J Biol Chem 278(37):34739-42
6) Nyswaner KM, et al.  (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
7) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64