LEM3/YNL323W Summary Help

Standard Name LEM3
Systematic Name YNL323W
Alias BRE3 , ROS3
Feature Type ORF, Verified
Description Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane (1, 2, 3)
Name Description Ligand-Effect Modulator 4
Chromosomal Location
ChrXIV:31944 to 33188 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LEM3 GO evidence and references
  View Computational GO annotations for LEM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All LEM3 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All LEM3 Interaction evidence and references
193 total interaction(s) for 108 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • PCA: 7
  • Two-hybrid: 1
Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 49
  • Phenotypic Enhancement: 36
  • Phenotypic Suppression: 17
  • Positive Genetic: 47
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2
Resources
Expression Summary
histogram
Resources
Protein Information All LEM3 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:31944 to 33188 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1245 31944..33188 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005267
References cited on this page View Complete Literature Guide for LEM3
1) Kato U, et al.  (2002) A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. J Biol Chem 277(40):37855-62
2) Hanson PK, et al.  (2003) Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine. J Biol Chem 278(38):36041-50
3) Lenoir G, et al.  (2009) Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter drs2p. J Biol Chem 284(27):17956-67
4) Sitcheran R, et al.  (2000) A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Genetics 156(3):963-72