LCD1/YDR499W Summary Help

Standard Name LCD1 1
Systematic Name YDR499W
Alias DDC2 2 , PIE1 3
Feature Type ORF, Verified
Description Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress (1, 3, 4)
Name Description Lethal, Checkpoint-defective, DNA damage sensitive 1
Chromosomal Location
ChrIV:1447830 to 1450073 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All LCD1 GO evidence and references
  View Computational GO annotations for LCD1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
reduction of function
unspecified
Resources
75 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 11
  • Biochemical Activity: 2
  • Co-localization: 2
  • PCA: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 9
  • Dosage Lethality: 3
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 8
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 7
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 747
Molecular Weight (Da) 86,424
Isoelectric Point (pI) 5.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1447830 to 1450073 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2244 1447830..1450073 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002907
References cited on this page View Complete Literature Guide for LCD1
1) Rouse J and Jackson SP  (2000) LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae. EMBO J 19(21):5801-12
2) Paciotti V, et al.  (2000) The checkpoint protein Ddc2, functionally related to S. pombe Rad26, interacts with Mec1 and is regulated by Mec1-dependent phosphorylation in budding yeast. Genes Dev 14(16):2046-59
3) Wakayama T, et al.  (2001) Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae. Mol Cell Biol 21(3):755-64
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76