LAS1/YKR063C Summary Help

Standard Name LAS1 1
Systematic Name YKR063C
Feature Type ORF, Verified
Description Protein required for pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability (1, 2, 3)
Name Description Lethal in the Absence of SSD1-v 1
Chromosomal Location
ChrXI:562297 to 560789 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All LAS1 GO evidence and references
  View Computational GO annotations for LAS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
overexpression
repressible
Large-scale survey
conditional
null
reduction of function
repressible
Resources
93 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 65
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 7
  • Co-fractionation: 3

Genetic Interactions
  • Negative Genetic: 6
  • Phenotypic Enhancement: 1
  • Positive Genetic: 3
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 502
Molecular Weight (Da) 59,351
Isoelectric Point (pI) 7.98
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:562297 to 560789 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1509 562297..560789 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001771
References cited on this page View Complete Literature Guide for LAS1
1) Doseff AI and Arndt KT  (1995) LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth. Genetics 141(3):857-71
2) Schillewaert S, et al.  (2012) The evolutionarily conserved protein Las1 is required for pre-rRNA processing at both ends of ITS2. Mol Cell Biol 32(2):430-44
3) Castle CD, et al.  (2013) Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae. Nucleic Acids Res 41(2):1135-50