Standard Name	LAG2 1
Systematic Name	YOL025W
Alias	ECM36
Feature Type	ORF, Verified
Description	Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 (1, 2, 3)
Name Description	Longevity Assurance Gene 4

Chromosomal Location	ChrXV:274957 to 276939 | ORF Map | GBrowse

Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	negative regulation of ubiquitin-protein ligase activity (IGI, IMP) replicative cell aging (IMP)
Cellular Component
High-throughput	mitochondrion (IDA)

Classical genetics
null	heat sensitivity: increased Aah1p accumulation: decreased Cdc53p modification: decreased replicative lifespan: decreased resistance to caffeine: decreased utilization of carbon source: decreased viable
overexpression	replicative lifespan: increased
Large-scale survey
null	competitive fitness: decreased haploproficient Rad52-YFP distribution: increased resistance to L-canavanine: increased viable
unspecified	resistance to Calcofluor White: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	42 total interaction(s) for 20 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-Western: 8 Co-fractionation: 1 Reconstituted Complex: 7 Two-hybrid: 5
Genetic Interactions	Dosage Growth Defect: 3 Negative Genetic: 6 Synthetic Growth Defect: 6 Synthetic Lethality: 1 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:274957 to 276939 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-07-27
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1983 274957..276939 2011-02-03 1997-07-27
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005385