Standard Name	KTI11 1, 2
Systematic Name	YBL071W-A
Alias	DPH3 3
Feature Type	ORF, Verified
Description	Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs (2, 4, 5, 6, 7, 8)
Name Description	Kluveromyces lactis Toxin Insensitive 9

Chromosomal Location	ChrII:89978 to 90226 | ORF Map | GBrowse

	View Computational GO annotations for KTI11
Molecular Function
Manually curated	electron carrier activity (IDA) iron ion binding (IDA) zinc ion binding (IDA)
Biological Process
Manually curated	peptidyl-diphthamide biosynthetic process from peptidyl-histidine (IMP) tRNA wobble uridine modification (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Pathways	diphthamide biosynthesis

Classical genetics
dominant negative	tRNA modification: abnormal toxin resistance: increased
null	heat sensitivity: increased killer toxin resistance: increased resistance to sordarin: increased resistance to caffeine: decreased resistance to paromomycin: decreased toxin resistance: increased
reduction of function	heat sensitivity: increased killer toxin resistance: increased resistance to caffeine: decreased toxin resistance: normal
unspecified	killer toxin resistance: increased toxin resistance: increased
Large-scale survey
null	acid pH resistance: decreased heat sensitivity: increased vegetative growth: decreased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	43 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 11 Affinity Capture-RNA: 1 Affinity Capture-Western: 15 Co-purification: 1 PCA: 5 Protein-RNA: 1 Two-hybrid: 1
Genetic Interactions	Positive Genetic: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 1 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:89978 to 90226 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 2001-01-30
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..249 89978..90226 2011-02-03 2001-01-30
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000007587