KRE28/YDR532C Summary Help

Standard Name KRE28 1
Systematic Name YDR532C
Feature Type ORF, Verified
Description Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation (1, 2, 3)
Chromosomal Location
ChrIV:1500553 to 1499396 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All KRE28 GO evidence and references
  View Computational GO annotations for KRE28
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
Large-scale survey
null
unspecified
Resources
69 total interaction(s) for 56 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 4
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 24
  • Positive Genetic: 12
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 385
Molecular Weight (Da) 44,674
Isoelectric Point (pI) 4.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1500553 to 1499396 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1158 1500553..1499396 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002940
References cited on this page View Complete Literature Guide for KRE28
1) Pagliuca C, et al.  (2009) Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. PLoS One 4(10):e7640
2) Nekrasov VS, et al.  (2003) Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(12):4931-46
3) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540