KNS1/YLL019C Summary

Standard Name	KNS1 1
Systematic Name	YLL019C
Feature Type	ORF, Verified
Description	Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation (1, 2, 3 and see Summary Paragraph) Also known as: L124
Name Description	Kinase Next to SPA2 1

Chromosomal Location	ChrXII:107899 to 105686 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: -28 cM

Gene Ontology Annotations All KNS1 GO evidence and references

	View Computational GO annotations for KNS1
Molecular Function
Manually curated	protein serine/threonine kinase activity (IDA, IMP) protein tyrosine kinase activity (IDA, IMP)
High-throughput	protein kinase activity (IDA)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase III promoter (IDA, IGI, IMP) protein autophosphorylation (IDA) protein phosphorylation (IDA)
High-throughput	protein phosphorylation (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA)

Regulatory Role

Regulatory modules	predicted: cellcycle (239)

Mutant phenotypes All KNS1 Phenotype evidence and references

Classical genetics
null	viable
Large-scale survey
null	auxotrophy competitive fitness: decreased filamentous growth: decreased haploinsufficient invasive growth: absent resistance to fluconazole: decreased resistance to bleomycin: decreased resistance to cycloheximide: decreased resistance to oleate: decreased resistance to tunicamycin: increased resistance to cycloheximide: increased resistance to tirapazamine: increased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All KNS1 Interaction evidence and references

	66 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 23 Affinity Capture-RNA: 1 Biochemical Activity: 7 Two-hybrid: 6
Genetic Interactions	Negative Genetic: 14 Phenotypic Enhancement: 1 Positive Genetic: 13 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All KNS1 Protein evidence and references

Localization	Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:107899 to 105686 | |

Note: this feature is encoded on the Crick strand.

This feature contains embedded feature(s): YLL019W-A

: -28 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2214	107899..105686	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003942

SUMMARY PARAGRAPH for KNS1

KNS1 encodes a member of the LAMMER family of protein kinases, a conserved family of eukaryotic kinases so named because they share the amino acid motif "LAMMER" (2). As with other LAMMER protein kinases, Kns1p would be classified as a serine/threonine kinase based on amino acid sequence, but is actually able to phosphorylate tyrosine as well (2). Kns1p has autophosphorylation activity in vitro (2). The carboxy-terminus of Kns1p has similarity to other protein kinases such as Cdc2p of S. pombe and Cdc28p of S. cerevisiae (1). Cells with KNS1 knocked out have no readily detectable phenotype (1).

Last updated: 1999-09-01

References cited on this page View Complete Literature Guide for KNS1

1)	Padmanabha R, et al. (1991) The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family. Mol Gen Genet 229(1):1-9
2)	Lee K, et al. (1996) Activity and autophosphorylation of LAMMER protein kinases. J Biol Chem 271(44):27299-303
3)	Lee J, et al. (2012) TOR signaling regulates ribosome and tRNA synthesis via LAMMER/Clk and GSK-3 family kinases. Mol Cell 45(6):836-43