Standard Name	KAR5 1
Systematic Name	YMR065W
Alias	FIG3 2
Feature Type	ORF, Verified
Description	Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone (2, 3, 4)
Name Description	KARyogamy

Chromosomal Location	ChrXIII:399702 to 401216 | ORF Map | GBrowse

	View Computational GO annotations for KAR5
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	karyogamy involved in conjugation with cellular fusion (IGI, IMP)
Cellular Component
Manually curated	endoplasmic reticulum membrane (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	mating efficiency: decreased Prm3p-GFP distribution: abnormal
overexpression	Prm3p-GFP distribution: abnormal
reduction of function	nuclear fusion during mating: abnormal
Large-scale survey
null	anaerobic growth: decreased competitive fitness: increased haploproficient resistance to bleomycin: increased resistance to camptothecin: increased resistance to hydroxyurea: increased resistance to tunicamycin: increased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to sirolimus: decreased resistance to cycloheximide: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	34 total interaction(s) for 27 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 1 Co-localization: 1 PCA: 1 Two-hybrid: 9
Genetic Interactions	Negative Genetic: 18 Phenotypic Enhancement: 1 Positive Genetic: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXIII:399702 to 401216 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1515 399702..401216 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004669