KAR3/YPR141C Summary Help

Standard Name KAR3 1
Systematic Name YPR141C
Alias OSR11
Feature Type ORF, Verified
Description Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description KARyogamy 1
Chromosomal Location
ChrXVI:817923 to 815734 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 94.4 cM
Gene Ontology Annotations All KAR3 GO evidence and references
  View Computational GO annotations for KAR3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
571 total interaction(s) for 285 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • Co-crystal Structure: 5
  • Co-purification: 1
  • PCA: 3
  • Reconstituted Complex: 9
  • Two-hybrid: 39

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 5
  • Negative Genetic: 292
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 18
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 138
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 729
Molecular Weight (Da) 84,003
Isoelectric Point (pI) 9.39
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:817923 to 815734 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 94.4 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2190 817923..815734 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006345
References cited on this page View Complete Literature Guide for KAR3
1) Meluh PB and Rose MD  (1990) KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60(6):1029-41
2) Page BD, et al.  (1994) Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol 124(4):507-19
3) Middleton K and Carbon J  (1994) KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Proc Natl Acad Sci U S A 91(15):7212-6
4) Mackey AT and Gilbert SP  (2003) The ATPase cross-bridge cycle of the Kar3 motor domain. Implications for single head motility. J Biol Chem 278(6):3527-35
5) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64