KAR3/YPR141C Summary

KAR3/YPR141C Summary

Standard Name	KAR3
Systematic Name	YPR141C
Alias	OSR11
Feature Type	ORF, Verified
Description	Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description	KARyogamy

Chromosomal Location	ChrXVI:817923 to 815734 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: 94.4 cM

Gene Ontology Annotations All KAR3 GO evidence and references

	View Computational GO annotations for KAR3
Molecular Function
Manually curated	minus-end-directed microtubule motor activity (IDA) protein heterodimerization activity (IPI)
Biological Process
Manually curated	karyogamy involved in conjugation with cellular fusion (IMP) meiosis (IDA, IMP) mitosis (IGI) mitotic sister chromatid cohesion (IGI, IMP) nuclear migration involved in conjugation with cellular fusion (IMP)
Cellular Component
Manually curated	cytoplasmic microtubule (IDA) microtubule (TAS) spindle pole body (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All KAR3 Phenotype evidence and references

Classical genetics
null	chromosome/plasmid maintenance: abnormal colony sectoring: increased nuclear position: abnormal resistance to cincreasin: decreased resistance to hydroxyurea: decreased
reduction of function	nuclear fusion during mating: abnormal
Large-scale survey
null	cell size: increased colony sectoring: increased competitive fitness: decreased heat sensitivity: increased resistance to hydroxyurea: decreased resistance to 5-fluorouracil: decreased resistance to ethanol: decreased resistance to sirolimus: increased resistance to fluconazole: increased resistance to quinine: decreased resistance to methyl methanesulfonate: decreased resistance to cycloheximide: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to sodium selenide: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All KAR3 Interaction evidence and references

	569 total interaction(s) for 286 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 4 Affinity Capture-Western: 8 Biochemical Activity: 1 Co-crystal Structure: 3 Co-purification: 1 PCA: 3 Reconstituted Complex: 9 Two-hybrid: 39
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 5 Negative Genetic: 292 Phenotypic Enhancement: 2 Phenotypic Suppression: 2 Positive Genetic: 18 Synthetic Growth Defect: 33 Synthetic Lethality: 138 Synthetic Rescue: 8
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All KAR3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:817923 to 815734 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

: 94.4 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2190	817923..815734	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006345

References cited on this page View Complete Literature Guide for KAR3

1)	Meluh PB and Rose MD (1990) KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60(6):1029-41
2)	Page BD, et al. (1994) Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol 124(4):507-19
3)	Middleton K and Carbon J (1994) KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore. Proc Natl Acad Sci U S A 91(15):7212-6
4)	Mackey AT and Gilbert SP (2003) The ATPase cross-bridge cycle of the Kar3 motor domain. Implications for single head motility. J Biol Chem 278(6):3527-35
5)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64