KAP95/YLR347C Summary Help

Standard Name KAP95 1
Systematic Name YLR347C
Alias RSL1
Feature Type ORF, Verified
Description Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p (1, 2, 3, 4, 5)
Name Description KAryoPherin 1
Chromosomal Location
ChrXII:826413 to 823828 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All KAP95 GO evidence and references
  View Computational GO annotations for KAP95
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
conditional
overexpression
repressible
unspecified
Large-scale survey
null
overexpression
reduction of function
Resources
298 total interaction(s) for 136 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 149
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 50
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 3
  • PCA: 6
  • Protein-peptide: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 46
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 2
  • Negative Genetic: 8
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 861
Molecular Weight (Da) 94,775
Isoelectric Point (pI) 4.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:826413 to 823828 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YLR347W-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2586 826413..823828 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004339
References cited on this page View Complete Literature Guide for KAP95
1) Enenkel C, et al.  (1995) Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J Biol Chem 270(28):16499-502
2) Aitchison JD, et al.  (1996) Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274(5287):624-7
3) Gilchrist D and Rexach M  (2003) Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J Biol Chem 278(51):51937-49
4) Mackinnon MA, et al.  (2009) The kap60-kap95 karyopherin complex directly regulates phosphatidylcholine synthesis. J Biol Chem 284(11):7376-84
5) Lonhienne TG, et al.  (2009) Importin-{beta} Is a GDP-to-GTP Exchange Factor of Ran: IMPLICATIONS FOR THE MECHANISM OF NUCLEAR IMPORT. J Biol Chem 284(34):22549-58