KAP114/YGL241W Summary Help

Standard Name KAP114 1
Systematic Name YGL241W
Feature Type ORF, Verified
Description Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress (2, 3, 4, 5, 6)
Name Description KAryoPherin 1
Chromosomal Location
ChrVII:45445 to 48459 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All KAP114 GO evidence and references
  View Computational GO annotations for KAP114
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
111 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 17
  • Biochemical Activity: 4
  • Protein-peptide: 2
  • Reconstituted Complex: 25
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 1
  • Negative Genetic: 15
  • Phenotypic Suppression: 3
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,004
Molecular Weight (Da) 113,920
Isoelectric Point (pI) 4.38
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:45445 to 48459 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3015 45445..48459 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003210
References cited on this page View Complete Literature Guide for KAP114
1) Pemberton LF, et al.  (1999) Nuclear import of the TATA-binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting. J Cell Biol 145(7):1407-17
2) Morehouse H, et al.  (1999) The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein. Proc Natl Acad Sci U S A 96(22):12542-7
3) Mosammaparast N, et al.  (2002) A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J 21(23):6527-38
4) Hodges JL, et al.  (2005) Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains. Mol Biol Cell 16(7):3200-10
5) Rothenbusch U, et al.  (2012) Sumoylation regulates Kap114-mediated nuclear transport. EMBO J 31(11):2461-72
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76