IXR1/YKL032C Summary Help

Standard Name IXR1 1
Systematic Name YKL032C
Alias ORD1 2
Feature Type ORF, Verified
Description Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Intrastrand cross (X)-link Recognition 1
Chromosomal Location
ChrXI:381858 to 380065 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IXR1 GO evidence and references
  View Computational GO annotations for IXR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 802 genes
Regulators 13 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
263 total interaction(s) for 221 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 187
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 3
  • Positive Genetic: 31
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 7
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 597
Molecular Weight (Da) 67,856
Isoelectric Point (pI) 8.45
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:381858 to 380065 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1794 381858..380065 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001515
References cited on this page View Complete Literature Guide for IXR1
1) Brown SJ, et al.  (1993) Ixr1, a yeast protein that binds to platinated DNA and confers sensitivity to cisplatin. Science 261(5121):603-5
2) Lambert JR, et al.  (1994) The ORD1 gene encodes a transcription factor involved in oxygen regulation and is identical to IXR1, a gene that confers cisplatin sensitivity to Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 91(15):7345-9
3) McA'Nulty MM and Lippard SJ  (1996) The HMG-domain protein Ixr1 blocks excision repair of cisplatin-DNA adducts in yeast. Mutat Res 362(1):75-86
4) Chow CS, et al.  (1994) HMG domain proteins induce sharp bends in cisplatin-modified DNA. Biochemistry 33(50):15124-30
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Vizoso-Vazquez A, et al.  (2012) Ixr1p and the control of the Saccharomyces cerevisiae hypoxic response. Appl Microbiol Biotechnol 94(1):173-84