ISW2/YOR304W Summary Help

Standard Name ISW2 1
Systematic Name YOR304W
Feature Type ORF, Verified
Description ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide (1, 2, 3, 4, 5, 6)
Name Description Imitation SWitch subfamily 1
Chromosomal Location
ChrXV:884514 to 887876 | ORF Map | GBrowse
Gene Ontology Annotations All ISW2 GO evidence and references
  View Computational GO annotations for ISW2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
329 total interaction(s) for 220 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 59
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8
  • Co-crystal Structure: 1
  • Co-localization: 5
  • Co-purification: 4
  • Reconstituted Complex: 2

Genetic Interactions
  • Negative Genetic: 179
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 3
  • Positive Genetic: 23
  • Synthetic Growth Defect: 27
  • Synthetic Lethality: 1
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 1,120
Molecular Weight (Da) 130,326
Isoelectric Point (pI) 6.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:884514 to 887876 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3363 884514..887876 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005831
References cited on this page View Complete Literature Guide for ISW2
1) Tsukiyama T, et al.  (1999) Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev 13(6):686-97
2) Gelbart ME, et al.  (2001) Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates. Mol Cell Biol 21(6):2098-106
3) Ruiz C, et al.  (2003) Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex. Microbiology 149(Pt 2):341-51
4) Fazzio TG, et al.  (2005) Two distinct mechanisms of chromatin interaction by the Isw2 chromatin remodeling complex in vivo. Mol Cell Biol 25(21):9165-74
5) Fischer C, et al.  (2009) Kinetic mechanism for single-stranded DNA binding and translocation by Saccharomyces cerevisiae Isw2. Biochemistry 48(13):2960-8
6) Yadon AN, et al.  (2013) DNA looping facilitates targeting of a chromatin remodeling enzyme. Mol Cell 50(1):93-103