ISW1/YBR245C Summary Help

Standard Name ISW1 1
Systematic Name YBR245C
Alias SGN2 2
Feature Type ORF, Verified
Description ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK (1, 3, 4, 5, 6, 7)
Name Description Imitation SWitch subfamily 1
Chromosomal Location
ChrII:711539 to 708150 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ISW1 GO evidence and references
  View Computational GO annotations for ISW1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
301 total interaction(s) for 168 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 101
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 45
  • Phenotypic Enhancement: 55
  • Phenotypic Suppression: 5
  • Positive Genetic: 9
  • Synthetic Growth Defect: 35
  • Synthetic Lethality: 14
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,129
Molecular Weight (Da) 131,101
Isoelectric Point (pI) 6.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:711539 to 708150 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3390 711539..708150 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000449
References cited on this page View Complete Literature Guide for ISW1
1) Tsukiyama T, et al.  (1999) Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev 13(6):686-97
2) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
3) Vary JC Jr, et al.  (2003) Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91
4) Morillon A, et al.  (2003) Isw1 chromatin remodeling ATPase coordinates transcription elongation and termination by RNA polymerase II. Cell 115(4):425-35
5) Mellor J and Morillon A  (2004) ISWI complexes in Saccharomyces cerevisiae. Biochim Biophys Acta 1677(1-3):100-12
6) Smolle M, et al.  (2012) Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange. Nat Struct Mol Biol 19(9):884-92
7) Vasicova P, et al.  (2013) Nuclear import of chromatin remodeler Isw1 is mediated by atypical bipartite cNLS and classical import pathway. Traffic 14(2):176-93