Standard Name	ISU1 1
Systematic Name	YPL135W
Alias	NUA1
Feature Type	ORF, Verified
Description	Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable; ISU1 has a paralog, ISU2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	IscU homolog 1

Chromosomal Location	ChrXVI:297553 to 298050 | ORF Map | GBrowse

	View Computational GO annotations for ISU1
Molecular Function
Manually curated	ferrous iron binding (IDA)
Biological Process
Manually curated	cellular iron ion homeostasis (IMP, ISS) iron-sulfur cluster assembly (IGI, IMP, ISS) tRNA wobble uridine modification (IGI)
Cellular Component
Manually curated	mitochondrial matrix (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	aconitase activity: decreased resistance to hydrogen peroxide: decreased respiratory growth: decreased
reduction of function	iron molecular entity accumulation: increased aconitase activity: decreased resistance to ferrous sulfate: decreased
Large-scale survey
null	competitive fitness: increased resistance to ciclopirox olamine: decreased resistance to acetic acid: increased viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	75 total interaction(s) for 50 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Co-fractionation: 2 Co-purification: 1 Protein-RNA: 1 Reconstituted Complex: 6 Two-hybrid: 5
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 37 Phenotypic Enhancement: 1 Positive Genetic: 4 Synthetic Growth Defect: 3 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:297553 to 298050 | ORF Map | GBrowse
	This feature contains embedded feature(s): YPL135C-A
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..498 297553..298050 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006056