ISU1/YPL135W Summary Help

Standard Name ISU1 1
Systematic Name YPL135W
Alias NUA1
Feature Type ORF, Verified
Description Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable; ISU1 has a paralog, ISU2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description IscU homolog 1
Chromosomal Location
ChrXVI:297553 to 298050 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ISU1 GO evidence and references
  View Computational GO annotations for ISU1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 13 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
repressible
Resources
79 total interaction(s) for 52 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Co-fractionation: 2
  • Co-purification: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 37
  • Phenotypic Enhancement: 1
  • Positive Genetic: 4
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 165
Molecular Weight (Da) 17,895
Isoelectric Point (pI) 10.37
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:297553 to 298050 | ORF Map | GBrowse
This feature contains embedded feature(s): YPL135C-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..498 297553..298050 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006056
References cited on this page View Complete Literature Guide for ISU1
1) Schilke B, et al.  (1999) Evidence for a conserved system for iron metabolism in the mitochondria of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(18):10206-11
2) Garland SA, et al.  (1999) Saccharomyces cerevisiae ISU1 and ISU2: members of a well-conserved gene family for iron-sulfur cluster assembly. J Mol Biol 294(4):897-907
3) Muhlenhoff U, et al.  (2003) Components involved in assembly and dislocation of iron-sulfur clusters on the scaffold protein Isu1p. EMBO J 22(18):4815-25
4) Ramazzotti A, et al.  (2004) Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae. FEBS Lett 557(1-3):215-20
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61