Standard Name	IST3 1
Systematic Name	YIR005W
Alias	SNU17 2
Feature Type	ORF, Verified
Description	Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing (2, 3, 4)
Name Description	Increased Sodium Tolerance 1

Chromosomal Location	ChrIX:364889 to 365335 | ORF Map | GBrowse

	View Computational GO annotations for IST3
Molecular Function
Manually curated	contributes_to first spliceosomal transesterification activity (IDA, IMP)
Biological Process
Manually curated	generation of catalytic spliceosome for first transesterification step (IMP) mRNA export from nucleus (IMP) mRNA splicing, via spliceosome (IDA, IPI) spliceosomal complex assembly (IMP)
Cellular Component
Manually curated	RES complex (IDA) U2 snRNP (IDA)

Classical genetics
null	heat sensitivity: increased mating response: abnormal vegetative growth: decreased rate viable
Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal budding pattern: abnormal cell shape: abnormal chitin deposition: decreased competitive fitness: decreased endocytosis: decreased fermentative growth: decreased rate haploinsufficient heat sensitivity: increased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to camptothecin: increased resistance to methyl methanesulfonate: increased resistance to caffeine: decreased resistance to Calcofluor White: normal resistance to mycophenolic acid: increased resistance to hygromycin B: decreased respiratory growth: decreased rate stress resistance: increased utilization of phosphorus source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	323 total interaction(s) for 197 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Co-purification: 1 Reconstituted Complex: 7 Two-hybrid: 5
Genetic Interactions	Negative Genetic: 193 Positive Genetic: 60 Synthetic Growth Defect: 23 Synthetic Lethality: 4 Synthetic Rescue: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIX:364889 to 365335 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..447 364889..365335 2011-02-03 1994-12-10
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001444