IST3/YIR005W Summary Help

Standard Name IST3 1
Systematic Name YIR005W
Alias SNU17 2
Feature Type ORF, Verified
Description Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids (2, 3, 4, 5)
Name Description Increased Sodium Tolerance 1
Chromosomal Location
ChrIX:364889 to 365335 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All IST3 GO evidence and references
  View Computational GO annotations for IST3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
323 total interaction(s) for 197 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Co-purification: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 193
  • Positive Genetic: 59
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 4
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 148
Molecular Weight (Da) 17,098
Isoelectric Point (pI) 5.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:364889 to 365335 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..447 364889..365335 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001444
References cited on this page View Complete Literature Guide for IST3
1) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2) Gottschalk A, et al.  (2001) A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol 21(9):3037-46
3) Wang Q and Rymond BC  (2003) Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus. Mol Cell Biol 23(20):7339-49
4) Spingola M, et al.  (2004) Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res 32(3):1242-50
5) Tuo S, et al.  (2012) Apparent defect in yeast bud-site selection due to a specific failure to splice the pre-mRNA of a regulator of cell-type-specific transcription. PLoS One 7(10):e47621