ISC1/YER019W Summary Help

Standard Name ISC1 1
Systematic Name YER019W
Feature Type ORF, Verified
Description Inositol phosphosphingolipid phospholipase C; mitochondrial membrane localized; hydrolyzes complex sphingolipids to produce ceramide; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance (1, 2, 3, 4)
Name Description Inositol phosphoSphingolipid phospholipase C 1
Chromosomal Location
ChrV:192797 to 194230 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ISC1 GO evidence and references
  View Computational GO annotations for ISC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 124 genes
Resources
Pathways
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
225 total interaction(s) for 172 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • PCA: 1
  • Protein-RNA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 97
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 6
  • Positive Genetic: 64
  • Synthetic Growth Defect: 28
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 477
Molecular Weight (Da) 53,940
Isoelectric Point (pI) 7.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:192797 to 194230 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1434 192797..194230 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000821
References cited on this page View Complete Literature Guide for ISC1
1) Sawai H, et al.  (2000) Identification of ISC1 (YER019w) as inositol phosphosphingolipid phospholipase C in Saccharomyces cerevisiae. J Biol Chem 275(50):39793-8
2) Betz C, et al.  (2002) ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae. Eur J Biochem 269(16):4033-9
3) Okamoto Y, et al.  (2002) Structural requirements for selective binding of ISC1 to anionic phospholipids. J Biol Chem 277(48):46470-7
4) Kitagaki H, et al.  (2009) ISC1-dependent Metabolic Adaptation Reveals an Indispensable Role for Mitochondria in Induction of Nuclear Genes during the Diauxic Shift in Saccharomyces cerevisiae. J Biol Chem 284(16):10818-30