Standard Name	ISC1
Systematic Name	YER019W
Feature Type	ORF, Verified
Description	Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance (1, 2, 3)
Name Description	Inositol phosphoSphingolipid phospholipase C 1

Chromosomal Location	ChrV:192797 to 194230 | ORF Map | GBrowse

	View Computational GO annotations for ISC1
Molecular Function
Manually curated	inositol phosphosphingolipid phospholipase activity (IDA)
Biological Process
Manually curated	ceramide biosynthetic process (IMP) response to salt stress (IMP) sphingolipid catabolic process (IDA)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) integral to mitochondrial outer membrane (IDA) mitochondrion (IDA)

Pathways	phospholipids degradation

Classical genetics
null	chronological lifespan: decreased oxidative stress resistance: decreased petite resistance to hydrogen peroxide: decreased resistance to camptothecin (CPT): decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased resistance to ethidium bromide: decreased respiratory growth: decreased rate toxin resistance: decreased utilization of carbon source: decreased
reduction of function	resistance to hydroxyurea: decreased
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to tunicamycin: decreased resistance to sorbate: decreased resistance to sirolimus: decreased resistance to methyl methanesulfonate: decreased resistance to wortmannin: normal resistance to hydroxyurea: decreased resistance to fenpropimorph: decreased resistance to caffeine: decreased resistance to camptothecin: decreased resistance to bleomycin: decreased resistance to 1,4-dithiothreitol: decreased resistance to fluconazole: decreased resistance to cycloheximide: decreased resistance to 5-fluorouracil: decreased resistance to citric acid: decreased sporulation: absent viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	211 total interaction(s) for 163 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 PCA: 1 Protein-RNA: 1 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 92 Phenotypic Enhancement: 11 Phenotypic Suppression: 6 Positive Genetic: 57 Synthetic Growth Defect: 28 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 4 Synthetic Rescue: 7
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrV:192797 to 194230 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1434 192797..194230 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000821