Standard Name	ISA2 1
Systematic Name	YPR067W
Feature Type	ORF, Verified
Description	Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations (2, 3, 4, 5)
Name Description	Iron Sulfur Assembly

Chromosomal Location	ChrXVI:682220 to 682777 | ORF Map | GBrowse

	View Computational GO annotations for ISA2
Molecular Function
Manually curated	iron ion binding (IDA)
Biological Process
Manually curated	biotin biosynthetic process (IGI, IMP) iron-sulfur cluster assembly (IGI, IMP)
Cellular Component
Manually curated	mitochondrial intermembrane space (IDA)
High-throughput	mitochondrion (IDA)

Classical genetics
null	auxotrophy iron molecular entity accumulation: increased chromosome/plasmid maintenance: abnormal Ptr2p-GFP distribution: abnormal resistance to DFD-VI-15: decreased respiratory growth: absent viable
Large-scale survey
null	cell size: decreased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased heat sensitivity: increased metal resistance: decreased nutrient utilization: decreased resistance to CTBT: decreased resistance to calcium dichloride: decreased resistance to elesclomol-Cu: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to ethanol: decreased resistance to doxorubicin: decreased resistance to farnesol: decreased respiratory growth: decreased rate respiratory growth: absent stress resistance: increased toxin resistance: increased toxin resistance: decreased utilization of carbon source: decreased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	19 total interaction(s) for 18 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Biochemical Activity: 4
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Synthetic Growth Defect: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXVI:682220 to 682777 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..558 682220..682777 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000006271