ISA2/YPR067W Summary Help

Standard Name ISA2 1
Systematic Name YPR067W
Feature Type ORF, Verified
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations (2, 3, 4, 5)
Name Description Iron Sulfur Assembly
Chromosomal Location
ChrXVI:682220 to 682777 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ISA2 GO evidence and references
  View Computational GO annotations for ISA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
20 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 4

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 185
Molecular Weight (Da) 20,876
Isoelectric Point (pI) 7.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:682220 to 682777 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..558 682220..682777 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006271
References cited on this page View Complete Literature Guide for ISA2
1) Vickery, L. E.  (1999) Unpublished
2) Jensen LT and Culotta VC  (2000) Role of Saccharomyces cerevisiae ISA1 and ISA2 in iron homeostasis. Mol Cell Biol 20(11):3918-27
3) Pelzer W, et al.  (2000) Mitochondrial Isa2p plays a crucial role in the maturation of cellular iron-sulfur proteins. FEBS Lett 476(3):134-9
4) Rodriguez-Manzaneque MT, et al.  (2002) Grx5 is a mitochondrial glutaredoxin required for the activity of iron/sulfur enzymes. Mol Biol Cell 13(4):1109-21
5) Muhlenhoff U, et al.  (2011) Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins. J Biol Chem 286(48):41205-16