ISA1/YLL027W Summary Help

Standard Name ISA1 1
Systematic Name YLL027W
Feature Type ORF, Verified
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins (2, 3, 4)
Name Description Iron Sulfur Assembly
Chromosomal Location
ChrXII:87403 to 88155 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ISA1 GO evidence and references
  View Computational GO annotations for ISA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
23 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Two-hybrid: 2

Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 250
Molecular Weight (Da) 27,675
Isoelectric Point (pI) 10.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:87403 to 88155 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..753 87403..88155 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003950
References cited on this page View Complete Literature Guide for ISA1
1) Vickery, L. E.  (1999) Unpublished
2) Jensen LT and Culotta VC  (2000) Role of Saccharomyces cerevisiae ISA1 and ISA2 in iron homeostasis. Mol Cell Biol 20(11):3918-27
3) Muhlenhoff U, et al.  (2002) Characterization of iron-sulfur protein assembly in isolated mitochondria. A requirement for ATP, NADH, and reduced iron. J Biol Chem 277(33):29810-6
4) Muhlenhoff U, et al.  (2011) Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins. J Biol Chem 286(48):41205-16