INO4/YOL108C Summary Help

Standard Name INO4
Systematic Name YOL108C
Feature Type ORF, Verified
Description Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain (1, 2)
Name Description INOsitol requiring
Chromosomal Location
ChrXV:111886 to 111431 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All INO4 GO evidence and references
  View Computational GO annotations for INO4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 88 genes
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
474 total interaction(s) for 320 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Co-localization: 1
  • Far Western: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 17

Genetic Interactions
  • Negative Genetic: 272
  • Phenotypic Enhancement: 1
  • Positive Genetic: 148
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 151
Molecular Weight (Da) 17,378
Isoelectric Point (pI) 10.21
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:111886 to 111431 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..456 111886..111431 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005468
References cited on this page View Complete Literature Guide for INO4
1) Schwank S, et al.  (1995) Yeast transcriptional activator INO2 interacts as an Ino2p/Ino4p basic helix-loop-helix heteromeric complex with the inositol/choline-responsive element necessary for expression of phospholipid biosynthetic genes in Saccharomyces cerevisiae. Nucleic Acids Res 23(2):230-7
2) Santiago TC and Mamoun CB  (2003) Genome expression analysis in yeast reveals novel transcriptional regulation by inositol and choline and new regulatory functions for Opi1p, Ino2p, and Ino4p. J Biol Chem 278(40):38723-30
3) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
4) Bachhawat N, et al.  (1995) Functional characterization of an inositol-sensitive upstream activation sequence in yeast. A cis-regulatory element responsible for inositol-choline mediated regulation of phospholipid biosynthesis. J Biol Chem 270(42):25087-95