INO2/YDR123C Summary

Standard Name	INO2 (see Nomenclature conflict Note)
Systematic Name	YDR123C
Alias	DIE1 , SCS1 1
Feature Type	ORF, Verified
Description	Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion (2, 3, 4, 5)
Name Description	INOsitol requiring 6

Chromosomal Location	ChrIV:699468 to 698554 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for INO2
Molecular Function
Manually curated	contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA, IMP, IPI)
Biological Process
Manually curated	phospholipid biosynthetic process (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP, IPI)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role

Binding motifs	Predicted INO2 Binding Site Locations
	CACATGC 7 [Search intergenic regions for CACATGC] CATGTGAAAT 4 [Search intergenic regions for CATGTGAAAT]

Regulatory modules	predicted: stressResponse (391)

Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Classical genetics
null	tellurium atom accumulation: decreased resistance to tellurite: decreased
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cellular morphology: abnormal
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased haploinsufficient lipid particle morphology: abnormal resistance to hydroxyurea: increased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to chloroquine: decreased sporulation: decreased starvation resistance: decreased stress resistance: increased vacuolar morphology: abnormal viable
overexpression	inviable resistance to sirolimus: decreased vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

	262 total interaction(s) for 163 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-Western: 6 Biochemical Activity: 1 Co-localization: 1 Far Western: 1 Reconstituted Complex: 8 Two-hybrid: 7
Genetic Interactions	Dosage Rescue: 4 Negative Genetic: 184 Phenotypic Enhancement: 2 Positive Genetic: 33 Synthetic Growth Defect: 9 Synthetic Lethality: 2 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:699468 to 698554 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..915	699468..698554	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002530

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
RTS1	Nomenclature conflict	SCS1 has been used in the literature to refer to both RTS1/YOR014W, which encodes a protein phosphatase 2A and INO2/YDR123C, which encodes a RNA Pol II transcription factor.
LCB2	Nomenclature conflict	SCS1 has been used to describe both INO2/YDR123C, a transcription factor involved in phospholipid synthesis, and LCB2/YDR062W, a serine C-palmitoyltransferase involved in sphingolipid biosynthesis.

1)	Hosaka K, et al. (1994) Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis. J Biochem 115(1):131-6
2)	Schuller HJ, et al. (1995) DNA binding site of the yeast heteromeric Ino2p/Ino4p basic helix-loop-helix transcription factor: structural requirements as defined by saturation mutagenesis. FEBS Lett 370(1-2):149-52
3)	Hammond CL, et al. (1993) INO2, a regulatory gene in yeast phospholipid biosynthesis, affects nuclear segregation and bud pattern formation. Cell Mol Biol Res 39(6):561-77
4)	Ambroziak J and Henry SA (1994) INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter. J Biol Chem 269(21):15344-9
5)	Gardenour KR, et al. (2004) Identification of novel dominant INO2c mutants with an Opi- phenotype. Mol Microbiol 52(5):1271-80
6)	Loewy BS and Henry SA (1984) The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes. Mol Cell Biol 4(11):2479-85
7)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104