IML3/YBR107C Summary Help

Standard Name IML3
Systematic Name YBR107C
Alias MCM19
Feature Type ORF, Verified
Description Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 (1, 2, 3, 4, 5)
Name Description Increased Minichromosome Loss 6
Chromosomal Location
ChrII:454530 to 453793 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All IML3 GO evidence and references
  View Computational GO annotations for IML3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All IML3 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All IML3 Interaction evidence and references
158 total interaction(s) for 82 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 28
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 8
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 3
  • Two-hybrid: 15
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Negative Genetic: 34
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 2
  • Positive Genetic: 4
  • Synthetic Growth Defect: 32
  • Synthetic Lethality: 19
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All IML3 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:454530 to 453793 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..738 454530..453793 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000311
References cited on this page View Complete Literature Guide for IML3
1) Ghosh SK, et al.  (2001) The IML3/MCM19 gene of Saccharomyces cerevisiae is required for a kinetochore-related process during chromosome segregation. Mol Genet Genomics 265(2):249-57
2) Pot I, et al.  (2003) Chl4p and iml3p are two new members of the budding yeast outer kinetochore. Mol Biol Cell 14(2):460-76
3) Ghosh SK, et al.  (2004) The Iml3 protein of the budding yeast is required for the prevention of precocious sister chromatid separation in meiosis I and for sister chromatid disjunction in meiosis II. Curr Genet 46(2):82-91
4) Fernius J and Marston AL  (2009) Establishment of cohesion at the pericentromere by the Ctf19 kinetochore subcomplex and the replication fork-associated factor, Csm3. PLoS Genet 5(9):e1000629
5) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
6) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702