IME4/YGL192W Summary Help

Standard Name IME4 1
Systematic Name YGL192W
Alias SPO8
Feature Type ORF, Verified
Description mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70) (1, 2, 3, 4, 5)
Name Description Inducer of MEiosis 1, 6
Chromosomal Location
ChrVII:142246 to 144048 | ORF Map | GBrowse
Gbrowse
Genetic position: -130 cM
Gene Ontology Annotations All IME4 GO evidence and references
  View Computational GO annotations for IME4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
14 total interaction(s) for 11 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 600
Molecular Weight (Da) 69,395
Isoelectric Point (pI) 7.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:142246 to 144048 | ORF Map | GBrowse
SGD ORF map
Genetic position: -130 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1803 142246..144048 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003160
References cited on this page View Complete Literature Guide for IME4
1) Shah JC and Clancy MJ  (1992) IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae. Mol Cell Biol 12(3):1078-86
2) Clancy MJ, et al.  (2002) Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene. Nucleic Acids Res 30(20):4509-18
3) Hongay CF, et al.  (2006) Antisense transcription controls cell fate in Saccharomyces cerevisiae. Cell 127(4):735-45
4) Bodi Z, et al.  (2010) Yeast targets for mRNA methylation. Nucleic Acids Res 38(16):5327-35
5) Dominissini D, et al.  (2012) Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485(7397):201-6
6) Kassir Y, et al.  (1988) IME1, a positive regulator gene of meiosis in S. cerevisiae. Cell 52(6):853-62