IME4/YGL192W Summary Help

Standard Name IME4 1
Systematic Name YGL192W
Alias SPO8
Feature Type ORF, Verified
Description mRNA N6-adenosine methyltransferase required for entry into meiosis; mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70) (1, 2, 3, 4, 5)
Name Description Inducer of MEiosis 1, 6
Chromosomal Location
ChrVII:142246 to 144048 | ORF Map | GBrowse
Gbrowse
Genetic position: -130 cM
Gene Ontology Annotations All IME4 GO evidence and references
  View Computational GO annotations for IME4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 111 genes
Regulators 79 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
14 total interaction(s) for 11 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Two-hybrid: 5

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 600
Molecular Weight (Da) 69,395
Isoelectric Point (pI) 7.99
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:142246 to 144048 | ORF Map | GBrowse
SGD ORF map
Genetic position: -130 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1803 142246..144048 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003160
References cited on this page View Complete Literature Guide for IME4
1) Shah JC and Clancy MJ  (1992) IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae. Mol Cell Biol 12(3):1078-86
2) Clancy MJ, et al.  (2002) Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene. Nucleic Acids Res 30(20):4509-18
3) Hongay CF, et al.  (2006) Antisense transcription controls cell fate in Saccharomyces cerevisiae. Cell 127(4):735-45
4) Bodi Z, et al.  (2010) Yeast targets for mRNA methylation. Nucleic Acids Res 38(16):5327-35
5) Dominissini D, et al.  (2012) Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485(7397):201-6
6) Kassir Y, et al.  (1988) IME1, a positive regulator gene of meiosis in S. cerevisiae. Cell 52(6):853-62